Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: Relationships between lncRNA AC010761.9 expression (ΔCt value) and patient clinical pathologic factors and serum tumor markers

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA tissues by lncRNA expression chip assay. T cancer tissues, N matched non-cancer tissues. Cluster analyses from the six GA and their paired non-GA tissues lncRNA chip results showed that LncRNA AC010761.9 was over-expressed in GA tissues compared with that in the paired non-GA tissues (mean increased fold = 2.01 times, p < 0.05)

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA tissues by quantified RT-PCR measurement. T cancer tissues, N matched non-cancer tissues. T versus N, p < 0.01. The data were from 145 cases of GA. The higher the ΔCt values, the lower the lncRNA AC010761.9 expression. Data were obtained from three independent tests

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA cell lines. The data were from three GA cell lines (MGC-803, BGC-823, and SGC-7901) and control cells (normal gastric cell line [GES-1]). GA cells versus control cells (all p < 0.05). The higher the ΔCt values, the lower the lncRNA AC010761.9 expression. Data were obtained from three independent tests

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Control, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: Relationships between lncRNA AC010761.9 expression (ΔCt value) and patient clinical pathologic factors analyzed by univariate and multivariate

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: The expression of lncRNA AC010761.9 showed a positive correlation with the expression of TRAF4 mRNA. r = 0.385 and p < 0.01 were obtained by Pearson correlation analysis

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: Scientific Reports

Article Title: Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori -related gastric cancer

doi: 10.1038/srep36442

Figure Lengend Snippet: KATO-III cells were incubated for 16 h with pooled DU-derived or GC-derived H. pylori (n = 5) at an MOI of 10, then were fixed and immunostained with FITC-labeled anti- H. pylori antibodies. The left panels show representative images and the right panel shows the quantified results expressed as the mean ± S.D. *p < 0.05. The data shown are representative of those obtained in three independent experiments.

Article Snippet: After 3 TBST washes, the array was assembled using a 3 × 7 well hybridization cassette (Arrayit Corporation, Sunnyvale, CA) and incubated sequentially in individual wells for 1 h at room temperature with serum (1:100 in 1% BSA in TBST), then, after 3 TBST washes, with Cy3-conjugated rabbit anti-human IgG + IgM + IgA antibodies (1:20000 in 1% BSA in TBST, Jackson Immunoresearch Laboratories).

Techniques: Incubation, Derivative Assay, Labeling

Journal: Scientific Reports

Article Title: Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori -related gastric cancer

doi: 10.1038/srep36442

Figure Lengend Snippet: ( A ) Left, The selected antigens were purified and printed on a slide for fabrication of the GC-related protein microarray designed for a 3 × 7 well cassette. Middle , Individual serum samples from 7 normal individuals, 7 DU patients, and 7 GC patients were applied to individual wells. Right , The arrays were then incubated with Cy3-labeled anti-human IgG + IgM + IgA antibodies (green). The left panel shows a representative image of the probed GC-related array, while the panels on the right show a representative well, indicated in red on the left panel; the top panel shows the layout of the well and the bottom panel shows the result. Each antigen was printed in triplicate on the chip. Four concentrations of anti-human IgG + IgM + IgA antibodies (18.75–150 μg/ml) were used as positive controls, buffer was used as a negative control, and Cy3-labeled anti-mouse IgG was used as landmark. ( B ) Comparison of the seroreactivity of the samples from normal individuals, DU patients, or GC patients (n = 7 per group) with the three GC-related antigens, OipA, BabA, and SabA detected as the intensity of fluorescence of the Cy3-labeled anti-human IgG + IgM + IgA antibodies. *p < 0.05.

Article Snippet: After 3 TBST washes, the array was assembled using a 3 × 7 well hybridization cassette (Arrayit Corporation, Sunnyvale, CA) and incubated sequentially in individual wells for 1 h at room temperature with serum (1:100 in 1% BSA in TBST), then, after 3 TBST washes, with Cy3-conjugated rabbit anti-human IgG + IgM + IgA antibodies (1:20000 in 1% BSA in TBST, Jackson Immunoresearch Laboratories).

Techniques: Purification, Microarray, Incubation, Labeling, Negative Control, Comparison, Fluorescence

Journal: Cancer Cell

Article Title: Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets

doi: 10.1016/j.ccell.2019.02.009

Figure Lengend Snippet:

Article Snippet: Migration was recorded every hour for 72 hr using the IncuCyte system (Essen Bioscience) and number of cells migrated was calculated using IncuCyte quantification software.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Chemotaxis Assay, Microarray, Software

Journal: Journal of Cellular and Molecular Medicine

Article Title: A novel antisense lncRNA NT5E promotes progression by modulating the expression of SYNCRIP and predicts a poor prognosis in pancreatic cancer

doi: 10.1111/jcmm.15718

Figure Lengend Snippet: LncNT5E is up‐regulated in pancreatic cancer (PC) tissues and cell lines. A, The heat map from our previous lncRNA microarray reflected the differentially expressed lncRNAs in PC and normal tissues. T represents PC tissue, and N represents normal pancreatic tissue. ENST00000421594 indicates lncNT5E. B, Relative expression of lncNT5E in 45 paired PC and adjacent normal tissues. LncNT5E expression from all tissues was normalized to 18S expression (ΔCT) and then compared with adjacent normal tissues and converted to the fold change (2 −ΔΔCT ). C, Relative expression of lncNT5E in different cell lines. Data are shown as fold change (2 −ΔΔCT ). * P < .05, ** P < .01, *** P < .001

Article Snippet: We used microarray detection (H1602063, Arraystar Human LncRNA 8 × 60 k v3.0 1‐color) to study lncRNAs in three pairs of PC and adjacent normal tissues.

Techniques: Microarray, Expressing